Jameson was born in Dublin.

Her father, Denis Brownell Murphy (died 1842), was a miniature and enamel painter. He moved to England in 1798 with his family, and eventually settled at Hanwell, near London.

At sixteen years of age, she became governess in the family of Charles Paulet, 13th Marquess of Winchester. In 1821 she was engaged to Robert Jameson. The engagement was broken off, and Anna Murphy accompanied a young pupil to Italy, writing in a fictitious character a narrative of what she saw and did. She gave this diary to a bookseller on condition of receiving a guitar if he secured any profits. Colburn ultimately published it as The Diary of an Ennuyée (1826), which attracted much attention. Anna Murphy was governess to the children of Edward Littleton, later know as Baron Hatherton, from 1821 to 1825, when she married Robert Jameson.

The marriage proved unhappy. In 1829, when Jameson was appointed puisne judge in the island of Dominica the couple separated without regret, and Jameson visited Continental Europe again with her father.

The first work which displayed her powers of original thought was her Characteristics of Women (1832).These analyses of William Shakespeare's heroines are remarkable for their delicacy of critical insight and fineness of literary touch. They are the result of a penetrating but essentially feminine mind, applied to the study of individuals of its own sex, detecting characteristics and defining differences not perceived by the ordinary critic and entirely overlooked by the general reader.

German literature and art had aroused much interest in the United Kingdom of Great Britain and Ireland, and Jameson paid her first visit to the German Confederation in 1833. The conglomerations of hard lines, cold colours and pedantic subjects which decorated Munich under the patronage of King Ludwig I of Bavaria, were new to the world, and Jameson's enthusiasm first gave them an English reputation.

In 1836, Jameson was summoned to Canada by her husband, who had been appointed chief justice of the province of Upper Canada. He failed to meet her at New York, and she was left to make her way alone in winter to Toronto. Here she began the travelogue of her journey, Winter Studies and Summer Rambles in Canada, which was later published in Britain in 1838. After eight months of travelling and writing in Canada, she felt it useless to prolong a life far from all ties of family happiness and opportunities for a woman of her class and education. Before leaving, she undertook a journey to the depths of the Indian settlements in Canada; she explored Lake Huron, and saw much of emigrant and Aboriginal life unknown to colonial travellers. She returned to Great Britain in 1837.

At this period Jameson began making careful notes of the chief private art collections in and near London.The result appeared in her Companion to the Private Galleries (1842), followed in the same year by the Handbook to the Public Galleries. She edited the Memoirs of the Early Italian Painters in 1845. In the same year she visited her friend Ottilie von Goethe. Her friendship with Annabella Byron, 11th Baroness Wentworth dates from about this time and lasted for some seven years; it was brought to an end apparently through the Baroness' unreasonable temper.

A volume of essays published in 1846 contains one of Jameson's best pieces of work, The House of Titian. In 1847 she went to Italy with her niece and subsequent biographer (Memoirs, 1878), Gerardine Bate (Mrs. Macpherson), to collect materials for the work on which her reputation rests...her series of Sacred and Legendary Art. The time was ripe for such contributions to the traveller's library. The Acta Sanctorum and the Book of the Golden Legend had had their readers, but no one had ever pointed out the connection between these tales and the works of Christian art. The way to these studies had been pointed out in the preface to Kugler's Handbook of Italian Painting by Sir Charles Eastlake, who had intended pursuing the subject himself.

Eventually he made over to Jameson the materials and references he had collected. She recognized the extent of the ground before her as a mingled sphere of poetry, history, devotion and art. She infected her readers with her own enthusiastic admiration; and, in spite of her slight technical and historical equipment, Jameson produced a book which thoroughly deserved its great success.

She also took a keen interest in questions affecting the education, occupations and maintenance of her own sex.Her early essay on The Relative Social Position of Mothers and Governesses was the work of one who knew both sides; and in no respect does she more clearly prove the falseness of the position she describes than in the certainty with which she predicts its eventual reform. To her we owe the first popular enunciation of the principle of male and female cooperation in works of mercy and education. In her later years she took up a succession of subjects all bearing on the same principles of active benevolence and the best ways of carrying them into practice. Sisters of charity, hospitals, penitentiaries, prisons and workhouses all claimed her interest...all more or less included under those definitions of "the communion of love and communion of labour" which are inseparably connected with her memory. To the clear and temperate forms in which she brought the results of her convictions before her friends in the shape of private lectures...published as Sisters of Charity (1855) and The Communion of Labour (1856)--may be traced the source whence later reformers and philanthropists took counsel and courage.

She left the last of her Sacred and Legendary Art series in preparation. It was completed, under the title of The History of Our Lord in Art, by Lady Eastlake.