Cloning Vectors A Laboratory Manual Author:P. H. Pouwels, B. E. Enger-Valk, William J. Brammar, W.J. Brammar, B.E. Enger-Valk, P.H. Pouwels Loose-leaf Binder. 'Cloning Vectors', the very first comprehensive compilation of the presently existing vectors used in cloning, was published in December 1985. As a laboratory manual, it serves in rapidly identifying a given vector best suited for a particular purpose. Because of the extremely rapid developments in the field, annual supplement... more »s are published (starting from 1986), which comprise newly identified vectors. The first two supplementary updates are now available.A large section of the manual is devoted to Escherichia coli, the most commonly used host for general cloning and manipulation of DNA sequences. However, other Gram-negative bacteria are also discussed, as are several Gram-positives, such as Bacillus subtilis, the genera Staphylococcus, Streptococcus and Streptomyces, the lower eukaryotic yeasts and fungi, and animal and plant cells in culture.Within each chapter on these host organisms, separate sections have been created for general a« less