Physiology Pathology Bacteriology Anatomy Author:Unknown Author Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: SECTlON lll. GENERAL PATHOLOGY. METHODS—MICKOSCOPIC DIAGNOSIS. £a id Diagnosis of Babies. When it is possible L. Frothingham1 recommends that the speci... more »mens be fixed imbedded, cut and stained. The areas selected are the Ammon's horn, the hippocampus major,—from the cere- helium. Following is the technique: (1) Small pieces of Ammon's horn and of gasserian ganglion are placed in Zenkers' fluid for four hours; (2) ninety-five per cent. alcohol for several hours; (3) absolute alcohol, one-half hour; (4) absolute alcohol and chloroform, equal parts, 20 to 30 minutes; (5) chloroform 20 to 30 minutes; (6) chloroform and paraffin saturated solution, warm, 20 to 30 minutes; (7) paraffin at 55 C. 30 minutes; (8) imbed; (9) cut; (10) float sections on water at 40 C. and fix on slide with glycerin albumen; (11) 30 minutes at 55 C.; (12) xylol, to remove paraffin; (13) absolute alcohol; (14) 95 per cent. alcohol; (15) saturated alcoholic solution of iodin, 5 to 10 minutes; (16) wash out iodin with 95 per cent. alcohol; (17) wash in water; (18) stain 15 to 30 minutes in equal parts Unna's stain, 5 per cent. aqueous eosin (Grubler, W. G.); (19) wash in water; (20) Unna's stain, 3 to 5 minutes; (21) water; (22) differentiate in 95 per cent. alcohol until desired color is obtained (using microscope from time to time); (23) absolute alcohol; (24) xylol; (25) balsam. Another method recommended by Frothingham is as follows: Sections are (1) dried in the air; (2) 95 percent. alcohol, 10 minutes; (3) iodin solution, 10 minutes; (4) (1) Jour, Mecl, Research, April, 1906, 95 per cent. alcohol, 10 minutes; (5) water; (6) stain as above. Or, sections are (1) dried and fixed with gentle heat; (2) saturated alcoholic eosin, 15 minutes; (3) wash in water; (4) Loffler's alkaline methylen blu...« less